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AIHS Seminar Term1 - Shared screen with speaker view
Alexandra I. Cristea
Title: "Single-cell transcriptomics uncovers a novel role for glia in thirst-directed behaviours"
Alexandra I. Cristea
Abstract:Thirst emerges from a range of cellular changes that ultimately motivate an animal to consume water. Although some thirst-responsive neuronal signals have been reported, the full complement of brain responses is unclear. Here we identify molecular and cellular adaptations in the brain using single-cell RNA-sequencing of water deprived Drosophila. Perhaps surprisingly, water deficiency primarily altered gene expression in glia, rather than neurons. I will describe the various analyses that enabled us to reach this conclusion, and explain some follow-up experiments that we performed to demonstrate that one of the glial genes regulated by thirst in astrocytes contributes to regulating water consumption via modulation of glutamatergic neurotransmission
Alexandra I. Cristea
Dr Vincent CrosetBiography:I obtained my PhD from the University of Lausanne (Switzerland), working on the evolution and function of a then novel family of chemosensory receptors, in the lab of Richard Benton. There, I traced the evolution of these receptors across the genomes of dozens of invertebrates, and found a function for one of them in tasting amino-acids. After graduating in 2013, I moved to the Centre for Neural Circuits and Behaviour at the University of Oxford to work with Scott Waddell on transcriptomic variability in the Drosophila brain. There, I generated the first atlas of single-cell transcriptomes from the fly brain, which I used to identify new genes that define cell types, and resolve transmitter co-expression and receptor assembly. I also used single-cell transcriptomics to address state-dependent gene expression changes induced by thirst.